Today I decided to test Catchart's hypotheses in the lab.
Hypotheses: Ascorbate reduces the disulfide bridges - that connect the light chain with the heavy chain in antibodies - to sulfhydryl groups. This makes the antibodies unable to bind to antigen; they become unprimed.
1) I filled to glasses - A and B - with a solution with B antibodies (one drop of "standard" antibody solution in 1mL 0.9% NaCl solution)
2) To both A and B I added 2 drops of a 5% solution of blood cells with antigen B. In the solution added to A I had put a pinch of sodium ascorbate.
3) Then I mixed them and put them both on a glass plate to study them in a microscope.
Conclusion: As expected much of the blood cells were agglutinated in the control, while in the test solution there was almost no degree of agglutination. (at most 2-4 blood cells attached to each other). Ascorbate clearly affects the antibodies ability to bind to antigen. If it is by the mechanism described by Catchart is unknown, but likely.
Code: Select all
| A | | B |
| | | |
| | | |
B blood cells B blood cells
B antibodies B antibodies
This was done during class (I study medicine) so I had to use what was available of resources. Just had to see for myself. I guess this far from proves anything, but it "gives a hint".
One day I'm going to perform an experiment with solutions matching physiological condition, write an article and hopefully get it published. Maybe Catchart will be proven right some day. This is a very important property of ascorbate. Too bad it goes unnoticed by the medical establishment.